What is PCR?

Steps in PCR

There are Three (3) major steps in PCR reactions:
Denaturation, Annealing, and Extension

1



Denaturation

Template DNA is denatured to separate the double stranded molecules bound together by weak hydrogen bonds in order for it to be copied. This process occurs by heating the sample to around 95°C.

2



Annealing

The primers "anneal" to the separated strands of the template DNA to start replication. This process occurs by lowering the temperature to around 55° C to 60° C

3



Extension

the DNA polymerase attaches itself to the primers as it moves along the target DNA to add complementary bases from the dNTPs. This process occurs by increasing the temperature to 72°